Coding

Part:BBa_K606037:Design

Designed by: Cyrille Pauthenier   Group: iGEM11_Paris_Bettencourt   (2011-09-18)


ComS gene for the ComK/ComS system in B. Subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The start and the stop codon had been changed for more standard codons in bacteria. This part is very small so it is very hard to clone. It is better to amplify it by PCR before moving it.


Source

Extracted by PCR from B. Subtilis PY79 strain, and cloned in pSB1C3.

References